FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41604650
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT77800.027.3799461
GTATCGA51450.025.294814
CGTATCG57450.024.0601273
GTCCGAT62850.020.729051
CCGTATC76050.019.8820692
GTCCTAT84400.019.0981541
CCCGTAT102600.018.4446261
CTCCGCT196250.017.9532361
TCCGATT113550.015.4934542
CGATTGG51850.015.4811854
GTCCGAA72250.015.2022651
GTCCGGT90450.015.0345921
CCGTTTA68050.014.7979883
GTTCCGT63950.014.7960811
CTCCGAT111350.014.6466411
CCGATTG66450.014.4394723
GTCCCGT112050.014.4278361
CTCCGGT128700.014.0390991
GTCGTTT55300.013.8431491
GTCGGTT102800.013.8296831
Produced by FastQC (version 0.11.5)