FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42134700
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT57800.019.3335741
GTCCGTT72400.018.7844981
CTCCGCT192850.016.7672331
GTATCGA49450.015.8484014
CCCGTAT86250.015.3270231
CGTATCG53100.015.2966093
CTCCGAT109800.015.114551
CTCCGGT127450.014.4765151
TCCGATT102550.014.3282752
GTTCGTT47900.013.997671
CCGTATC77350.013.4634062
GTCCTAT84750.013.1856131
GTCGCAT35050.013.1599991
GTCCGAA74800.013.0959731
GTCCGCT97150.012.9710531
CCGATTG57100.012.8940453
CTCCGTT125400.012.8550651
GTCCCGT95400.012.7603831
CTCTGAT340900.012.5960071
GTCCGGT73650.012.5256751
Produced by FastQC (version 0.11.5)