FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42418968
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA42800.022.3058874
TGTTACG45200.020.5972333
TTACGAC50900.019.22285
TACGACT51700.018.6497366
GTCCGTT75500.014.6739441
GTCCGAT68700.014.119241
GTATCGA48600.013.2913724
CTCCGCT244450.012.915641
AGTGCGT115150.012.60169840-41
CCCGTAT98150.012.4018821
CGTTCGA115350.012.3332144-45
GTCCGGT82850.011.9947631
CTCCGGT149400.011.362021
ACGACTT91150.011.3075957
CGCAAGT130000.011.14391436-37
GTCCCGT108950.010.8233671
GTCGTTT52600.010.66680051
TAAGCCG77900.010.6088118
CGTATCG71150.010.548083
GTCGGAT56750.010.3894781
Produced by FastQC (version 0.11.5)