FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37765307
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG14250.017.83280240-41
CGCGTAT14100.017.68621844-45
TATGCCG62800.017.43437248-49
GTCCGTT52950.017.4222181
CGTATGC63500.017.35437646-47
TCGTATG52100.016.27403544-45
ATCGCGT16550.015.49796642-43
CGCTAGT12500.014.8361861
CTCCGCT134000.014.5849421
GTCCGGT47700.014.4549741
CTCCGGT92200.013.82202151
CTCCGAT87850.013.8027651
CTCCCGT93400.013.4407871
GTCCGAT48850.013.1412561
CTCCGTT103300.012.8891841
CCCGTAT58100.012.6859581
GTCCCGT65950.012.5459091
GTCCGAA50350.012.3719881
CTCCGAA75650.012.3201171
CTCGTAT71050.012.00038244-45
Produced by FastQC (version 0.11.5)