FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37396244
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92900.022.36945348-49
CGCGTAT20550.021.2651844-45
CGTATGC97800.021.1029946-47
TATCGCG22050.020.24882540-41
ATCGCGT22200.020.00497642-43
TCGTATG80750.019.88232844-45
CTCGTAT101750.016.17565344-45
CTCCGGT89100.015.9577731
CTCCCGT95700.015.6025821
GTATGCC137150.015.44657246-47
TATCTCG113050.014.5163740-41
TCGCGTA30650.014.41225242-43
GCCGTCT147750.014.30625250-51
CTCCGAT78000.013.839171
CCCGTAT53600.013.6626431
GTCCGTT51350.013.6130571
ATGCCGT157850.013.496181548-49
TGCCGTC161900.013.20258650-51
CGCTAGT13050.013.1180951
CTCCGTT106900.012.8557951
Produced by FastQC (version 0.11.5)