FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44987244
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT643870.14312279276321083TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG143950.028.34486248-49
CGTATGC146500.027.88389446-47
CGCGTAT33000.027.0605344-45
TATCGCG34250.026.6269840-41
TCGTATG119700.026.60706544-45
ATCGCGT35450.025.85944442-43
CTCGTAT142000.022.4119144-45
GTATGCC191650.021.57508746-47
TCGCGTA44000.020.9964142-43
TATCTCG156150.020.5173540-41
GCCGTCT204400.020.24096950-51
ATGCCGT216550.019.3794448-49
CTCCCGT120300.019.1712971
TGCCGTC220000.018.97842850-51
ACGAGTG232900.017.5185132-33
GCGTATG56350.017.06960544-45
CGAGTGG246100.016.74292832-33
CTCCGGT107350.016.389851
TCTCGTA195150.016.38040442-43
CTCCGAT105850.016.2627161
Produced by FastQC (version 0.11.5)