FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42041115
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT703520.16734094707050468TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG143350.029.97135748-49
CGTATGC145950.029.37234746-47
TCGTATG118400.028.6846744-45
AATCGCG33650.027.7368940-41
CGCGTAT34700.026.28253644-45
CGTACGT167250.025.80089234-35
CTCGTAT136750.024.81820944-45
GTACGTA176800.024.64902534-35
TACGTAA178900.024.12097536-37
CCGTACG181550.024.10873832-33
CGTAATC179600.024.09309438-39
ACGTAAT182300.023.78835536-37
GTATGCC184600.023.60858346-47
ACCGTAC190000.022.84904132-33
ATCGCGT44800.020.62147942-43
ATGCCGT223000.019.92665748-49
GCCGTCT217250.019.74334150-51
CACCGTA228700.019.2005730-31
TGCCGTC234500.018.69612150-51
CTCCCGT123550.017.3194081
Produced by FastQC (version 0.11.5)