FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34144492
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT366820.10743167594937421TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG46900.037.9785740-41
CGTTTCG246200.034.60090632-33
TTTCGGA268550.031.43810534-35
TTCGGAA280300.029.84970536-37
GTCACGT283550.029.79974228-29
AGTCACG282550.029.65305528-29
ACGTTTC286250.029.42793732-33
TCGGAAT293100.028.72439836-37
GTTTCGG296050.028.54189334-35
CGGAATC302300.027.92869838-39
CACGTTT339950.025.212330-31
AATCTCG264400.025.20440140-41
ATCGCGT32000.023.8232942-43
TCACGTT354550.023.75879130-31
ATCGCGG43250.023.55688542-43
GAGCACA751550.022.6684869
CTCGTAT80300.022.47836544-45
TCGTATG49550.021.76100244-45
AGAGCAC799050.021.41018
CGTGAAA89800.020.62923462-63
Produced by FastQC (version 0.11.5)