FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37386938
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG15750.024.12620540-41
TATGCCG70950.024.1015448-49
CGTATGC72500.023.68452646-47
TCGTATG56750.023.43616744-45
CGCGTAT17750.021.54229444-45
ATCGCGT20200.019.39904842-43
CTCGTAT74800.017.65377644-45
TATCTCG82000.016.39276340-41
GTATGCC111550.015.69142346-47
AGTCACG127200.013.70415228-29
TTATCGC29150.013.52443540-41
GTCACGT131050.013.28342828-29
GTCCGTT54050.012.8444791
CGCTAGT13800.012.7491711
CTCCCGT106400.012.6921751
TCACGTG143600.012.42025430-31
CCCGTAT50700.012.0987661
CTCCGAT86750.011.78496551
GCCGTCT149550.011.78369750-51
GTCCGAT53300.011.687011
Produced by FastQC (version 0.11.5)