FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40773221
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1423470.34911884935458004TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG223150.037.0807446-47
TCGTATG186650.036.5173942-43
CGTATGC228050.036.19027344-45
CTCGTAT205350.033.36544842-43
CGCGTAT44400.031.7188642-43
GTATGCC267050.030.93172544-45
CGGCTAC282950.028.99877432-33
ACGGCTA285550.028.9592932-33
GCCGTCT304250.027.22782748-49
ATGCCGT310800.026.70750246-47
TGCCGTC322950.025.99688148-49
ATCGCGT56250.024.7835840-41
AGTCACG340350.024.32438528-29
TCTCGTA291000.023.70020740-41
GTCACGG356050.023.31850228-29
TCACGGC360900.022.94596530-31
GCGTATG62700.022.80209742-43
CTACATC373250.022.32069636-37
ATCTCGT315000.022.12065740-41
CCGTCTT390900.021.5628650-51
Produced by FastQC (version 0.11.5)