FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40688610
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97200.022.96827946-47
CGTATGC101400.021.8061344-45
TCGTATG83350.020.37280342-43
CGCGTAT24750.019.47920242-43
CTCGTAT100950.017.36202442-43
TATCGCG27450.017.0442138-39
GTATGCC141150.015.69884244-45
TATCTCG113950.014.98537338-39
GTCCGAT54100.014.5904091
GTCCGTT56400.014.1640311
ATCGCGT34650.013.63961340-41
CTCCGAT93550.013.5713841
GCCGTCT165400.013.5551248-49
GTCCGGT57350.013.2660991
TGCCGTC183700.012.73485448-49
CCCGTAT54800.012.6686111
GTCCCGT71450.012.4450331
ATGCCGT180950.012.41650646-47
CTCCGCT162150.012.4045231
CTCCCGT112650.012.3256071
Produced by FastQC (version 0.11.5)