FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42763497
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC32703117.647435849317937TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC5859081.3701124582959154TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA6346400.060.1302729
AGAGCAC6520100.058.5647858
AAGAGCA6712350.056.959977
CGGAAGA6902950.055.4629174
GAAGAGC6966700.054.9513056
TCGGAAG7016650.054.553053
ATCGGAA7169100.053.3730552
GATCGGA7274600.052.6148831
GGAAGAG7314250.052.456435
TATGCCG4178300.045.4860946-47
CGTATGC4213000.045.08044444-45
TCGTATG3578600.045.05395542-43
GCCGTCT4213850.044.9112848-49
GTATGCC4234000.044.88097444-45
CTCGTAT3608100.044.7158742-43
ATGCCGT4251300.044.679946-47
TGCCGTC4255900.044.6645348-49
CGCGTAT649700.044.109642-43
CCGTCTT4293650.044.0051250-51
CGTCTTC4335050.043.5454250-51
Produced by FastQC (version 0.11.5)