FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30178724
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC20834076.903562257966904TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC3538721.1725876813081957TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC2511050.8320596987467064TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC2022480.6701674994608785TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC1402540.4647446326756559TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC557090.18459693657028045TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG483470.16020226700108328TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTGC457550.15161343468332195TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATTC302580.10026268837608907TruSeq Adapter, Index 8 (97% over 48bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4687950.071.928739
AGAGCAC4773300.070.691358
AAGAGCA4895300.068.989867
CGGAAGA4974750.068.002614
GAAGAGC5042950.067.047176
TCGGAAG5047750.067.011273
ATCGGAA5125150.065.9360052
GATCGGA5190800.065.120631
GGAAGAG5274950.064.172175
TATGCCG2593650.046.17059346-47
CGTATGC2608750.045.98619544-45
TCGTATG2288450.045.9612742-43
GTATGCC2626700.045.63577344-45
CTCGTAT2594600.045.62729342-43
GCCGTCT2200800.045.32245348-49
TGCCGTC2601200.045.2559348-49
CGCGTAT431900.045.24923342-43
ATGCCGT2462050.045.13873746-47
AATCGCG589500.045.0611738-39
CGTGAAA900000.044.95116860-61
Produced by FastQC (version 0.11.5)