FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44687405
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC6605121.4780719533837332TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC1200250.2685879835716574TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG963950.040.05713346-47
CGTATGC996950.038.71452744-45
TCGTATG844200.038.31053542-43
GCCGTCT1021050.037.6565348-49
GTATGCC1027600.037.4858444-45
CTCGTAT877300.037.06001742-43
ATGCCGT1039600.037.0348746-47
TGCCGTC1046800.036.97299248-49
CCGTCTT1081900.035.68772550-51
CGCGTAT174250.034.9049542-43
CGTCTTC1124450.034.2042150-51
TCTCGTA990350.032.77454840-41
ATCTCGT1004700.032.4293540-41
GCGTATG192250.031.64922142-43
CTTGAAA1223550.031.42214460-61
GCTTGAA1227150.031.3337158-59
TGCTTGA1250600.030.87531358-59
ATCGCGT197300.030.8031740-41
CATCTCG1074300.030.20896138-39
TCGCGTA207400.029.25730740-41
Produced by FastQC (version 0.11.5)