FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36558176
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2826910.7732634144548131TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC465790.12741062354970883TruSeq Adapter, Index 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG495400.033.8559646-47
CGTATGC522900.032.02546744-45
TCGTATG453950.031.8243342-43
GCCGTCT518200.031.09225348-49
GTATGCC541700.030.88331844-45
ATGCCGT553750.030.06116146-47
TGCCGTC559650.030.00313848-49
CTCGTAT488500.029.8068642-43
CCGTCTT577950.028.13664450-51
CGCGTAT83250.027.70029642-43
CGTCTTC610950.026.4147250-51
AGGGGGG588250.025.88818268-69
TCTCGTA575750.025.30645640-41
ATCTCGT599250.024.53201940-41
ACGATCT606200.024.27811836-37
GATCTCG614150.024.0798938-39
CACGATC716050.023.93993836-37
GCGTATG96600.023.77381142-43
CTTGAAA669200.023.44474460-61
TCACGAT732300.023.2884234-35
Produced by FastQC (version 0.11.5)