FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44606377
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT3394020.7608822388780869TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT951300.2132654709885988TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG565150.036.96466448-49
CGTATGC586550.035.6200846-47
CGCGTAT131900.035.52610444-45
ATCGCGT135700.034.5999242-43
TCGTATG471900.034.24361844-45
GTATGCC623250.033.46162446-47
GCCGTCT630750.032.86779850-51
TCGCGTA143000.032.6177342-43
ACGATCG145050.032.36975538-39
CTCGTAT505050.032.14174744-45
TGCCGTC653350.032.10164650-51
CGATCGC148200.031.777940-41
ATGCCGT659600.031.74720848-49
GCGTATG148000.031.72563244-45
GATCGCG150350.031.3550740-41
CCGTCTT665750.031.2360652-53
CGTCTTC710100.029.22832352-53
AACGATC725550.028.83759338-39
TCTCGTA570950.028.45157242-43
AAACGAT763350.027.27583136-37
Produced by FastQC (version 0.11.5)