FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37628147
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1945190.5169507815519058TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTAT646950.17193246321696362TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG335300.039.070948-49
CGTATGC349600.037.35723546-47
CGCGTAT86500.036.5996144-45
TCGTATG267200.035.9271144-45
CTCGTAT292300.033.93079844-45
GCCGTCT383050.033.37579750-51
GTATGCC385300.033.21169346-47
TGCCGTC406750.032.4704350-51
ATCGCGT98050.032.28740342-43
ATGCCGT407550.031.46846648-49
GCGTATG105300.029.90732644-45
CCGTCTT449700.029.26875952-53
CGCACAT455600.027.7475236-37
CGTCCGC469700.026.99510632-33
ATCTCGT371150.026.9391142-43
CCGCACA486500.026.90289336-37
GTCCGCA484650.026.8778634-35
TCTCGTA364800.026.54217342-43
ACGTCCG494500.026.40007232-33
CGTCTTC487550.026.3194352-53
Produced by FastQC (version 0.11.5)