FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48822616
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2173570.4451973650899821TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT678820.1390380228703845TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG403400.035.75467748-49
CGCGTAT97650.035.12044544-45
CGTATGC418300.034.39020546-47
ATCGCGT101250.033.7296342-43
TATCGCG101600.033.6135940-41
TCGTATG332300.032.80555344-45
TCGCGTA107050.031.96870242-43
GTATGCC466450.030.8605946-47
GCCGTCT465400.030.83826450-51
CTCGTAT362700.030.33095244-45
TGCCGTC492850.029.46764450-51
ATGCCGT497300.029.041748-49
CCGTCTT501300.028.7906852-53
GCGTATG121150.028.64124344-45
GTATCGC123050.027.86989240-41
TATCTCG396350.027.49722740-41
CCGTATC534950.026.77922838-39
TCCGTAT540700.026.37143536-37
CGTCTTC554200.026.03395552-53
CGTATCT422150.025.77734838-39
Produced by FastQC (version 0.11.5)