FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_dei019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_dei019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34808761
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT559730.1608014717903921TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG127350.027.75052548-49
CGTATGC136100.026.0536746-47
TCGTATG113300.025.34332744-45
CGCGTAT27850.024.3897944-45
CTCGTAT130100.022.16198344-45
AATCGCG30750.021.85723140-41
ATCGCGT31850.021.32589742-43
GTATGCC168250.021.17402546-47
CGCTAGT12200.019.097451
GCCGTCT184400.019.08765850-51
ATGCCGT201900.017.8802948-49
AATCTCG164100.017.72888240-41
TCGCGTA38200.017.5943742-43
TGCCGTC210250.017.2378850-51
TCTCGTA179200.016.39390442-43
CCGTCTT219550.016.25874952-53
ATCTCGT192650.015.20004142-43
GCGTATG45850.014.91833544-45
CCCGTAT56300.014.7798091
CGTCTTC266250.013.58538352-53
Produced by FastQC (version 0.11.5)