FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20865456
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9092054.357465276579625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT26800.09.3928141
CGGGAAT40650.09.0506591
CGGGGAT47650.08.7370071
CGCGTAT8852.1931319E-68.2049341
CGACGAT19350.08.0056421
CGGGCAT45700.07.94460341
CCGGAAT40800.07.83088641
CGGAAAT44550.07.7150331
GGGATAT59050.07.70610861
CGGGAAC53450.07.6983631
CGGGCAC76050.07.63851931
GCCGGTT32700.07.55004551
GATCGGA194550.07.51493394-95
CTCGATT26600.07.4615561
GGGAAAT115900.07.4347051
CCGGGAT44950.07.43098831
GGGGAAT79350.07.32084941
ATCGGAA197650.07.313009794-95
CGGAATA35250.07.27029372
CCCACAT147250.07.2654771
Produced by FastQC (version 0.11.5)