FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_FDK287_LM3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_FDK287_LM3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19269562
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA135800.011.120884
CCGGAAT46400.010.0480391
CGGGGAT52350.09.9056511
CGGGAAT46750.09.7692861
CCGGTTT48550.09.701061
CATCTAC145700.09.4851235
CGCAAAT23200.09.2277921
CGGGCAT49900.09.1525881
ATCTACA143050.09.0634456
CCCACAT165450.09.0576811
GTCGGTT36600.08.9689431
AAATCGA36800.08.775524
GTCGGAT23900.08.7584651
CGCCGAT29350.08.753041
CCGGTAT17400.08.7493131
TAGCGAT23650.08.6351179
GGGAAAT128800.08.6062361
TACAGAC138600.08.5665859
CCGGGAT54450.08.5625161
GTTCGAT26850.08.5049191
Produced by FastQC (version 0.11.5)