FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_FDK262_LM1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_FDK262_LM1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30460277
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA204650.010.0718284
CGGGGAT82100.09.8500461
CCGGAAT67400.09.7398361
CGGGAAT65800.09.5429031
CCGGTTT77900.08.91554451
CCGGGAT85400.08.9123981
GGGAAAT216450.08.9008181
CGGAAAT68700.08.7246151
GTCGGAT38850.08.69360351
CGGCGAT36650.08.4366861
GTCGGTT50350.08.3141081
CGCGGAT29250.08.2942441
CGCGAAT22400.08.2822641
GATCGGA404350.08.26996394-95
GCGGAAT39350.08.220471
ATCTACA221350.08.1746666
CGACGAT31850.08.0652311
CGGTTTC99650.08.0615482
CATCTAC223000.08.028945
ATCGGAA393700.07.95075594-95
Produced by FastQC (version 0.11.5)