FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_FDK241_LM2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_FDK241_LM2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29944266
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT42350.011.7943441
CGGGGAT77550.09.87601951
CGGGAAT68600.09.8469581
CGGGCAT79400.09.7058231
ACATCTA204350.09.5522894
GTTCGAT43900.09.4274141
CCGGGAT75900.09.4012881
GTCGGTT59750.09.3946791
CGCCGAT47650.09.2844871
CCGGTTT77950.09.2760981
CCGGAAT77400.09.2190921
CGGAAAT75450.09.0160161
CTCGAAT75700.08.86055851
GGAAAAT295000.08.498191
ATCTACA210900.08.3995896
GCCGAAT39850.08.3561761
CGGCAAT41650.08.337691
GGCGTAT42300.08.3220291
GGGAAAT205050.08.1894141
CGAATGT64700.08.1503283
Produced by FastQC (version 0.11.5)