FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_FDK236_LP1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_FDK236_LP1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26613498
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA171900.010.8027434
CGGGAAT61400.09.9165351
AAATCGA58550.09.2472174
CATCTAC194700.09.025355
CGGGTAT26500.08.9751651
GCGGAAT39500.08.9115441
ATCTACA196600.08.8415146
CGGGCAT75450.08.8264711
CGGCAAT41850.08.7521281
AATCGAG42350.08.522925
CCGGAAT61950.08.44636251
CGCGGAT28750.08.4382171
GGGAAAT191000.08.4178591
CCGGGAT77250.08.3744981
CGGGGAT74550.08.2949551
GGCGAAT35200.08.2433841
CTCGAAT59800.08.0341241
CGGAAAT63600.08.0028561
GGGGAAT121700.07.97366431
CCCACAT226550.07.95780851
Produced by FastQC (version 0.11.5)