FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4601_AM4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4601_AM4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29347992
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA206200.011.147574
GCGGAAT37000.010.9284461
CGGGAAT69500.010.4724391
CGGGGAT75400.010.4100731
CGCCGAT58600.09.9850151
ATCTACA213650.09.669456
CATCTAC217800.09.5288155
CCGGAAT69150.09.4247451
CGGCGAT30900.09.3910161
CGCGGAT24350.09.3774231
CCGGGAT75850.09.2821241
CCCACAT249750.08.9141841
CCGGGTT58800.08.8993151
CGGAAAT69350.08.8488021
CCGGTTT73050.08.6610921
TACAGAC209000.08.6353249
GCCGGTT50750.08.6236881
CGGGCAT77950.08.6048681
CGCGGGT38800.08.5823761
AATCGAG40400.08.3462695
Produced by FastQC (version 0.11.5)