FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4599_ADI5_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4599_ADI5_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29998330
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT46550.010.4228941
CGGGGAT78900.010.2489591
CCGGAAT75000.010.084251
GCGGAAT44350.010.0818911
GTTCGAT42200.09.9192321
CGGAAAT75200.09.3616341
CGCGGAT28950.09.2012591
CTCGAAT70850.08.9293451
CGACAAT39650.08.8776161
CGGGCAT79500.08.8552811
CGGGAAT70650.08.7526391
GGCGTAT40300.08.6163951
CGGGTAT32050.08.60810851
TTCGATT46400.08.6052722
CCGGCAT66350.08.4595821
GATCGGA404950.08.39852794-95
ACATCTA195700.08.3966254
CGGAATT60100.08.3836812
CGGCGAT35200.08.3783181
CGAATGT61550.08.2581283
Produced by FastQC (version 0.11.5)