FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4597_AP5_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4597_AP5_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32200939
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT48550.010.19051551
CGGGAAT70600.09.837851
CGGGGAT85150.09.3859161
ACATCTA204650.09.1901934
GCGGAAT43600.09.056151
CGGCGAT38550.08.5148451
CGCCGAT58100.08.3517231
CCGGAAT75350.08.27065851
CCGGGAT86500.08.2494921
CGGAAAT75700.08.2324191
GCCGAAT47800.08.1608991
GCGGGTT53150.08.1449821
GTCGGAT43250.08.1394981
GATCGGA373900.08.09233394-95
CGCAATT31300.08.0553341
CGCGAAT18900.08.054531
GGAAAAT316750.08.0350611
CCGGTTT69500.08.0085351
CTCGAAT70800.07.995871
CGACGAT33950.07.9870631
Produced by FastQC (version 0.11.5)