FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4595_ADI4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4595_ADI4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30687359
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT70150.010.3074291
GTTCGAT45250.010.30248451
CCGGAAT82700.010.2387971
GCGGAAT46650.010.1972451
CCGGGAT77450.010.1343761
GGCGTAT44250.09.8902891
CGGGCAT84100.09.5027161
GTCGGTT57600.09.3323371
CGGGGAT75300.09.1602541
TTCGATT54600.09.1417182
CGCAAAT39550.08.780331
CGCGGAT27100.08.776781
CGGAATT64950.08.6364252
GGAAAAT293600.08.4738371
CGCCGAT44000.08.3247751
GCGGGTT45500.08.2594311
CGGAAAT76950.08.2221
GGCGAAT37650.08.2126411
GCGGATT55050.08.2091981
GCCGGTT55600.08.1279921
Produced by FastQC (version 0.11.5)