FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4593_AP4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4593_AP4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31535853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT72500.09.9734521
GGCGAAT41100.09.72248651
CGGCAAT48350.09.4452761
CGGGGAT83500.09.4002061
GCGGAAT43500.09.2954321
CCGGGAT84200.09.265561
CGACAAT40400.08.9489511
AATCGAG42000.08.7070575
CGGGCAT83550.08.6544011
CGACGAT32400.08.3689261
CGGCGAT36500.08.341171
CGCGGAT27950.08.3397721
CCGGAAT79900.08.3352991
AAATCGA62800.08.2434934
GATCGGA432200.08.17108294-95
CGGGTAT35550.08.0288171
GGGAAAT222500.08.017541
TCGGATC71650.07.96154642
CTCGAAT72400.07.9503591
GTCGGTT57000.07.92845631
Produced by FastQC (version 0.11.5)