FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4579_AM3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4579_AM3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30943231
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA224350.011.1565764
CGCCGAT46650.010.7068561
CGGGAAT67350.010.311921
ATCTACA233300.09.7102816
CCGGAAT71250.09.6807151
CGGGGAT82150.09.6122451
CATCTAC228700.09.4487135
CGGAAAT71350.09.0671171
CCCACAT269050.08.9462651
GCGGAAT40500.08.9265331
CGGGCAT81850.08.6594811
AATCGAG43800.08.5660465
CGCAAAT36250.08.3983941
GCCGGTT56300.08.3647131
GGCGAAT37500.08.2452981
GATCGGA349950.08.19820594-95
CCGGTTT79200.08.1684191
CGGCAAT47800.08.0608611
TAGCGAT39000.08.03760059
CGCGAAT20750.08.0236911
Produced by FastQC (version 0.11.5)