FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4567_ADI3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4567_ADI3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31469512
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT88900.09.8466521
CGGAAAT77750.09.7290241
CGGGAAT70850.09.6693041
GCCGAAT40900.09.6544461
CCGGAAT80550.09.5680191
ACATCTA210850.09.3476944
CGGCAAT52150.09.1225921
CGAAAAT76600.09.067691
GGCGAAT40650.09.0116181
CGGGGAT79800.08.9425411
CCGGGAT82750.08.7387271
CGCCGAT49950.08.6671921
GTCGGTT59100.08.5327871
GGGAAAT213050.08.5077751
CGGGTAT32100.08.4477761
CGGAATT61550.08.4186162
GATCGGA405950.08.371860594-95
CTCGAAT78550.08.1763631
CGGCGAT35000.08.1555971
GGCGTAT42000.08.1555971
Produced by FastQC (version 0.11.5)