FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4565_AP3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4565_AP3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23917514
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT40150.09.9508861
CGGCAAT39650.09.5965421
CCGGGAT68400.09.45694351
GCGGAAT36250.09.4469691
CGGGAAT54950.09.4346711
CGGGGAT69100.08.9481511
GGCGAAT33100.08.7653621
CCGGAAT58800.08.5742581
GCGGATT44450.08.56024551
CGGAAAT61150.08.5558711
CGACGAT25450.08.4099361
CTCGAAT55450.08.3202851
GGGGAAT115550.08.1501051
GGGAAAT169900.08.0904461
ACATCTA146350.08.0804884
CGAATCG14305.4569682E-117.97274543
TCGGATC51350.07.960222
GATCGGA302550.07.91272594-95
CTCGGAT80200.07.76899671
CGCGAAT16603.6379788E-127.7361291
Produced by FastQC (version 0.11.5)