FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4478_AP2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4478_AP2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31815002
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT85050.010.6827991
CGGGAAT78200.09.7328331
CGGCAAT58550.09.668211
CGGAAAT79600.08.9042881
CGGGGAT97600.08.870491
ACATCTA194100.08.8088334
GCGGAAT51100.08.6574121
CCGGGAT97850.08.6533681
CGCGAAT24950.08.1983031
GGGAAAT229350.08.0889451
AAATCGA63150.07.9721224
ATCTACA209250.07.8985566
GGGGAAT158850.07.78595921
GGAAAAT297400.07.6456241
AGATCGG478900.07.612306694-95
CGCGGAT34200.07.5109291
CGGAATT62500.07.4532682
CGGGCAT93900.07.4469381
GAAATCG86000.07.40221743
GATCGGA422800.07.38658294-95
Produced by FastQC (version 0.11.5)