FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4476_AM2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4476_AM2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30633130
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA206400.011.5279684
ATCTACA231950.010.0328996
CGCCGAT48700.09.9637921
CATCTAC234250.09.8329075
CGGGGAT82150.09.4970721
CGGCGAT32600.09.1934051
CGGGAAT66100.09.0682311
CCCACAT262950.08.9915931
CGCGGAT31650.08.8681241
TAGCGAT41950.08.8309459
ACCTTAC97600.08.71019656
CCGGAAT67350.08.6880241
CCGGTTT72050.08.6494961
GGGAAAT215000.08.5851271
AAATCGA65800.08.5168864
AATCGAG49250.08.4858665
GGCGAAT38250.08.4572851
TACAGAC218400.08.3724529
CGGAAAT68700.08.2403131
GGGGAAT136950.08.1979151
Produced by FastQC (version 0.11.5)