FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4474_ADI2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4474_ADI2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33493426
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT78350.010.4426761
CGACGAT33950.09.9481171
ACATCTA211850.09.6399864
CGGGAAT75050.09.5074251
CGCCGAT49700.09.4754791
GTTCGAT42500.09.4018361
CCGGGAT96750.08.89917851
CGGGGAT90500.08.8830161
GCGGAAT46700.08.7599961
TAGCGAT40750.08.5082219
GGGAAAT231200.08.4562211
CGGCAAT53500.08.3578851
GATCGGA426650.08.33309694-95
ATCTACA240850.08.1240526
GGCGAAT43550.08.0828751
GCGTCGT165250.08.0051694-95
CGGGCAT97750.07.9808551
AGATCGG485650.07.9515894-95
GCGGGTT57150.07.90732771
CGGAAAT75250.07.77536771
Produced by FastQC (version 0.11.5)