FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4460_ADI1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4460_ADI1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33002074
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT82500.010.3214151
GTTCGAT48550.09.8962871
CGCCGAT54850.09.8003591
CGGGGAT89950.09.7838721
GGCGAAT42900.09.7581061
CCGGGAT92300.09.483231
ACATCTA207600.09.0593014
GCGGAAT47950.08.928821
CGGGAAT77300.08.7387441
CCGGTTT75850.08.6549321
CGAATGT68400.08.6122163
CGGAAAT85400.08.5783361
CTCGAAT82150.08.5702661
CGGAATT64700.08.5226692
GCCGAAT45800.08.5170361
GTCGGTT61400.08.4449741
CGAAAAT77700.08.3264141
GGGAAAT228600.07.990891
GATCGGA395600.07.984586794-95
TTCGATT59200.07.94941852
Produced by FastQC (version 0.11.5)