FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4450_AM1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4450_AM1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31414823
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA216400.011.5877844
GCGGAAT39200.011.5278311
ATCTACA236850.010.2663186
CATCTAC244550.09.9234755
CGGGAAT68600.09.8463281
ACCTTAC95900.09.5577766
CCCACAT271650.09.4031781
TACAGAC236000.09.2975739
CGCCGAT58750.08.906251
GGGAAAT225150.08.8310921
CCGGAAT73250.08.7667011
CGGGGAT82850.08.6695091
GGCGTAT36100.08.4330141
CGGGCAT88350.08.399011
GCCGGTT59500.08.3143151
CGGCAAT46700.08.2504611
CGCGGTT29200.07.98221251
CCGGTTT71050.07.96696571
CGGAAAT69300.07.9622321
CCGGGAT83950.07.9326341
Produced by FastQC (version 0.11.5)