FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n02_DG4446_AP1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n02_DG4446_AP1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31209170
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT34900.09.9512221
CGGGAAT70000.09.8548271
ACATCTA204500.09.6613544
CGGGGAT86400.09.6361451
CGCGGAT29850.09.56281951
CCGGGAT86200.09.4377371
CGGCGAT36250.08.9244181
GGCGTAT36850.08.9082141
GCGGAAT46550.08.7893711
ATCTACA214650.08.7174346
CCGGAAT81400.08.708451
GTCGGTT57800.08.6425221
CGGCAAT52350.08.6334821
CGCCGAT54250.08.5941981
CGAATCG17700.08.5890443
GCGGGTT50450.08.5814221
GTTCGAT42400.08.5275991
GGCGAAT39150.08.506391
CATCTAC220100.08.5015655
GTCGGAT39250.08.4847181
Produced by FastQC (version 0.11.5)