FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20865456
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12055525.777740970530431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC98500.033.219599
ATCGGAA306700.029.7640042
GATCGGA302450.029.2377451
CGGAAGA316350.029.1018834
TCGGAAG321750.028.2300553
AGAGCAC330200.027.931078
TTCCCTA119100.027.3544398
CGTCTGA166750.026.82962216-17
GAGCACA350050.026.3876559
ACGTCTG171000.025.99575814-15
ACACGTC180250.024.97753312-13
CACGTCT184350.024.15187614-15
TACACGA68300.022.91137512-13
CACACGT201150.022.51215712-13
CGACGCT69450.022.43009416-17
CTCGTAT59750.022.09707544-45
TTCCGAT66250.021.50718524-25
AAGAGCA448650.021.0649197
CCGATTG34900.021.02561426-27
CACTCTT153850.021.0033822
Produced by FastQC (version 0.11.5)