FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_FDK287_LM3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_FDK287_LM3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19269562
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT240640.12488088727704347TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50200.031.74462148-49
CTCGTAT48850.027.51706344-45
AATCTCG60850.025.5641940-41
TCGTATG62100.024.8583344-45
CGTATGC63150.024.7458746-47
TCTCGTA59650.022.77381342-43
AATCGCG12450.022.70016140-41
CGTCTGA152950.021.33518816-17
ATCTCGT71550.020.9776942-43
ACGTCTG163250.020.01812214-15
ATCGGAA326950.019.9344772
ATGCCGT75250.019.75691648-49
ACACGTC168000.019.50855412-13
GAGCACA344650.019.4875879
GATCGGA335050.019.312071
CGGAAGA343350.019.3006294
AGAGCAC347800.019.215598
GTATGCC87000.018.8902246-47
CACGTCT178250.018.42683414-15
TCGGAAG360450.018.187213
Produced by FastQC (version 0.11.5)