FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_FDK262_LM1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_FDK262_LM1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30460277
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT717560.23557238169567532TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG117750.036.08303548-49
TATCTCG121850.033.8943540-41
CTCGTAT120500.033.72222544-45
TCGTATG131250.033.20396844-45
CGTATGC137200.031.4870446-47
TCTCGTA135450.029.84240242-43
ACCGCTC169850.028.3147532-33
ATCTCGT153250.027.02708842-43
CGCTCAT181100.026.5689434-35
ATGCCGT159550.026.21296548-49
TCACCGC191600.025.3237430-31
CCGCTCA198400.024.4437232-33
GTCACCG201700.024.18533328-29
CACCGCT203850.023.9184630-31
TGCCGTC168700.023.77762850-51
GTATGCC183350.023.53570746-47
GCCGTCT160900.022.55387950-51
CCGTCTT165900.022.07455452-53
CGTCTGA233050.021.52315116-17
CATTATC215900.020.33936938-39
Produced by FastQC (version 0.11.5)