FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_FDK241_LM2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_FDK241_LM2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29944266
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT799470.2669860066030672TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG138900.037.73602748-49
CTCGTAT135250.035.17215744-45
TCGTATG158200.033.2373644-45
CGAGATT183100.032.2194332-33
TCTCGTA136950.031.80479842-43
CGTATGC167450.031.7133446-47
GTCACGA193350.030.83085828-29
TCACGAG198250.030.27238530-31
ATGCCGT174550.029.40295448-49
ACGAGAT202750.029.34279432-33
AGTCACG203900.029.34046628-29
ATCTCGT153800.028.567442-43
CACGAGA216600.027.62004330-31
TGCCGTC179600.026.98941650-51
GTATGCC199700.026.5799946-47
CCGTCTT172200.025.44598652-53
GCCGTCT175200.025.02386750-51
CGTCTGA263850.023.05218916-17
GAGCACA555850.022.072759
ATCGGAA543850.022.0205972
Produced by FastQC (version 0.11.5)