FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_FDK236_LP1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_FDK236_LP1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26613498
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT266440.1001146110143056TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60650.028.38982448-49
CGTATGC69150.024.38491646-47
TCGTATG67800.024.2399644-45
CTCGTAT58900.023.83020244-45
AATCTCG79000.021.0138740-41
TCTCGTA72450.019.37334442-43
CGTCTGA180100.018.73879216-17
ACGTCTG191850.017.56634314-15
ATCTCGT87900.017.53522542-43
AATCGCG16500.017.41635940-41
ACACGTC193400.017.27808412-13
ATGCCGT96700.017.21658748-49
ATCGGAA389650.017.0682532
GATCGGA401150.016.5086691
CACGTCT207400.016.26074414-15
CGGAAGA419350.016.0633184
GAGCACA443200.015.82939159
TCGGAAG426200.015.7939693
AGAGCAC444400.015.4554548
TGCCGTC105150.014.9521750-51
Produced by FastQC (version 0.11.5)