FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4601_AM4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4601_AM4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29347992
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT588760.20061338438418547TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG106800.035.29059248-49
CTCGTAT104700.032.66405544-45
TCGTATG119450.031.99073644-45
CGTATGC127700.030.1657146-47
CGAGATT141650.030.11233932-33
GTCACGA158500.027.34572428-29
TCTCGTA113500.027.18125342-43
TCACGAG162050.026.7173330-31
ACGAGAT162250.026.36232432-33
AGTCACG167950.025.8212128-29
ATGCCGT144900.025.43762448-49
CACGAGA172400.025.00315730-31
ATCTCGT125150.024.46122742-43
GTATGCC170450.022.71141646-47
TGCCGTC158150.022.1801750-51
CGTCTGA202800.021.52471716-17
GCCGTCT147700.021.38576350-51
CCGTCTT149050.021.1761352-53
ACGTCTG218000.019.95852714-15
ATCGGAA443850.019.4043542
Produced by FastQC (version 0.11.5)