FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4599_ADI5_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4599_ADI5_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29998330
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG349100.11637314477172561TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG128650.030.3860840-41
ATCGGGG135600.027.5149942-43
CACTCCG187300.027.21099530-31
AGAATCG158150.026.3699938-39
GAATCGG151650.026.34136440-41
ACTCCGG210950.024.23910732-33
CCGGAGA221200.023.07296634-35
CGTCTGA235450.022.18122516-17
ACGTCTG250200.020.98743414-15
CGGAGAA242800.020.75633436-37
ATCGGAA506550.020.7159612
CTCCGGA254300.020.4806832-33
TCGGGGG150950.020.45328542-43
ACACGTC256150.020.43490412-13
GATCGGA512450.020.3767051
CGGAAGA522100.020.3264334
GAGCACA530700.020.3260029
TCCGGAG257200.020.19435334-35
AGAGCAC535250.020.0201728
CACGTCT272500.019.27864614-15
Produced by FastQC (version 0.11.5)