FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4597_AP5_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4597_AP5_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32200939
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT815160.25314789733305604TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG142250.036.14610748-49
TCGTATG147350.034.86274744-45
TACTCGA155000.034.33713536-37
CGTATGC152650.034.04126746-47
CTCGTAT133400.033.6303544-45
ACTCGAT147950.033.35680436-37
TTACTCG168700.032.91420434-35
TCTCGTA138350.030.67603942-43
ATGCCGT179300.028.62400248-49
ATCTCGT156000.026.53546142-43
CTCGATC165150.025.85621838-39
TGCCGTC189200.025.8458750-51
TCGATCT165050.025.48337438-39
ATTACTC224450.024.96101434-35
GCCGTCT184200.024.85840850-51
GTATGCC212450.024.85066246-47
CCGTCTT183750.024.76414952-53
CGATCTC170200.024.68437640-41
GATCTCG171750.024.47543540-41
ACATTAC243650.023.21823532-33
Produced by FastQC (version 0.11.5)