FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4595_ADI4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4595_ADI4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30687359
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT923570.3009610569615978TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG159150.038.51545748-49
TACTCGA175850.035.99218436-37
CTCGTAT149200.035.75158744-45
ACTCGAT163150.035.7369736-37
TTACTCG185000.035.5085634-35
TCGTATG176200.034.57290344-45
CGTATGC186000.033.24926846-47
TCTCGTA152550.032.58452642-43
ATGCCGT194050.031.33141748-49
ATCTCGT165900.029.4327742-43
CTCGATC178950.028.36135138-39
TGCCGTC204850.028.31160250-51
ATTACTC234950.028.28296534-35
GTATGCC220900.027.98546446-47
GCCGTCT193900.027.95069550-51
CCGTCTT195000.027.70779252-53
CGATCTC180500.027.3283740-41
GATCTCG180000.027.12720140-41
TCGATCT181650.027.08996238-39
ATCGGAA540500.024.7061122
Produced by FastQC (version 0.11.5)