FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4593_AP4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4593_AP4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31535853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT535960.16995259332290774TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG104200.033.55020548-49
TCGTATG117100.030.01651244-45
CTCGTAT105300.029.59108544-45
CGTATGC123500.028.74945846-47
CGAGATT140900.027.50822432-33
TCTCGTA112400.025.65118642-43
GTCACGA155400.025.38481728-29
ATGCCGT137550.025.10492348-49
TCACGAG159250.025.0394430-31
AGTCACG166350.023.85662828-29
ACGAGAT164000.023.80736532-33
CACGAGA176650.022.3041730-31
ATCTCGT129500.022.11732742-43
TGCCGTC156300.020.98410650-51
GTATGCC174050.020.64525846-47
CCGTCTT151700.019.89824152-53
GCCGTCT152100.019.86157650-51
CGTCTGA223250.017.93597416-17
ACGTCTG234450.017.04878414-15
ACACGTC237050.016.76150912-13
Produced by FastQC (version 0.11.5)