FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4579_AM3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4579_AM3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30943231
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT574820.1857659919224337TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG99100.033.71908648-49
TATCTCG104650.030.97767340-41
CTCGTAT104300.030.37566844-45
TCGTATG112350.029.95374344-45
CGTATGC119200.028.15268946-47
TCTCGTA116200.027.1627642-43
ACCGCTC145600.025.88627432-33
ATCTCGT132700.024.39382742-43
CGCTCAT154100.024.28888334-35
ATGCCGT136550.023.81042348-49
TCACCGC171150.022.25786830-31
CACCGCT176700.021.62596930-31
GTCACCG177400.021.59425528-29
CCGCTCA178000.021.46792432-33
GTATGCC166000.020.7450246-47
TGCCGTC152300.020.49045450-51
GCCGTCT144950.019.6943850-51
CGTCTGA199900.019.62716516-17
CCGTCTT149600.019.06634752-53
ACGTCTG212150.018.39305114-15
Produced by FastQC (version 0.11.5)