FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4567_ADI3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4567_ADI3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31469512
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT723940.23004487645057856TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG130450.036.12022848-49
CTCGTAT128450.033.317744-45
TCGTATG146900.032.30189544-45
CGTATGC153600.031.23300646-47
CGAGATT174800.030.27096732-33
TCTCGTA130650.030.22995442-43
GTCACGA183050.029.1534328-29
TCACGAG188100.028.5347230-31
ACGAGAT192750.027.69839132-33
AGTCACG193950.027.51500928-29
ATGCCGT168400.027.47259948-49
ATCTCGT144150.027.25056642-43
CACGAGA205150.026.12847130-31
GTATGCC195400.024.66102646-47
TGCCGTC180250.024.59929550-51
GCCGTCT171350.023.576250-51
CCGTCTT173600.023.33899952-53
CGTCTGA237250.022.71376616-17
ACGTCTG251900.021.43047314-15
ATCGGAA509900.020.8875432
Produced by FastQC (version 0.11.5)